Inorganic Carbon Accumulation by Chlamydomonas reinhardtii

Effect of Carbonic Anhydrase Inhibitors on Inorganic Carbon Accumulation by Chlamydomonas reinhardtii.

Membrane-permeable and impermeable inhibitors of carbonic anhydrase have been used to assess the roles of extracellular and intracellular carbonic anhydrase on the inorganic carbon concentrating system in Chlamydomonas reinhardtii. Acetazolamide, ethoxzolamide, and a membrane-impermeable, dextran-bound sulfonamide were potent inhibitors of extracellular carbonic anhydrase measured with intact c...

Inorganic Carbon Accumulation by Chlamydomonas reinhardtii: New Proteins are made During Adaptation to Low CO(2).

When the unicellular green alga Chlamydomonas reinhardtii is placed under low CO(2) conditions it adapts by making an inorganic carbon accumulating mechanism. Algal cells were labeled with (35)SO(4) (-2) during this adaptation period and labeled proteins specific for this low CO(2) adaptation were identified. Four major proteins were preferentially synthesized under low CO(2) conditions and had...

Oil accumulation is controlled by carbon precursor supply for fatty acid synthesis in Chlamydomonas reinhardtii.

Microalgal oils have attracted much interest as potential feedstocks for renewable fuels, yet our understanding of the regulatory mechanisms controlling oil biosynthesis and storage in microalgae is rather limited. Using Chlamydomonas reinhardtii as a model system, we show here that starch, rather than oil, is the dominant storage sink for reduced carbon under a wide variety of conditions. In s...

Chlamydomonas reinhardtii

A mutation at the PFlO locus of the unicellular green alga Chlamydomonas reinhardtii leads to abnormal cell motility. The asymmetric form of the ciliary beat stroke characteristic of wild-type flagella is modified by this mutation to a nearly symmetric beat. We report here that this abnormal motility is a conditional phenotype that depends on light intensity. In the absence of light or under lo...

Proposed carbon dioxide concentrating mechanism in Chlamydomonas reinhardtii.